Plant Pests of the Middle East


Publications by year


Publications by Authors

entomology authors

Recent Publications

Contact Us

The Department of Entomology
The Robert H. Smith Faculty of Agriculture, Food and Environment
The Hebrew University of Jerusalem
Herzl 229, Rehovot 7610001, ISRAEL

Tel: 08-9489223 
Fax: 08-9366768

Cross-Species Y Chromosome Function Between Malaria Vectors of the Species Complex


Bernardini, F. ; Galizi, R. ; Wunderlich, M. ; Taxiarchi, C. ; Kranjc, N. ; Kyrou, K. ; Hammond, A. ; Nolan, T. ; Lawniczak, M. N. K. ; Papathanos, P. A. ; et al. Cross-Species Y Chromosome Function Between Malaria Vectors of the Species Complex. Genetics 2017, 207, 729-740.

Date Published:

2017 10


Y chromosome function, structure and evolution is poorly understood in many species, including the genus of mosquitoes-an emerging model system for studying speciation that also represents the major vectors of malaria. While the Anopheline Y had previously been implicated in male mating behavior, recent data from the complex suggests that, apart from the putative primary sex-determiner, no other genes are conserved on the Y. Studying the functional basis of the evolutionary divergence of the Y chromosome in the gambiae complex is complicated by complete F1 male hybrid sterility. Here, we used an F1 × F0 crossing scheme to overcome a severe bottleneck of male hybrid incompatibilities that enabled us to experimentally purify a genetically labeled Y chromosome in an background. Whole genome sequencing (WGS) confirmed that the Y retained its original sequence content in the genomic background. In contrast to comparable experiments in , we find that the presence of a heterospecific Y chromosome has no significant effect on the expression of genes, and transcriptional differences can be explained almost exclusively as a direct consequence of transcripts arising from sequence elements present on the Y chromosome itself. We find that Y hybrids show no obvious fertility defects, and no substantial reduction in male competitiveness. Our results demonstrate that, despite their radically different structure, Y chromosomes of these two species of the gambiae complex that diverged an estimated 1.85 MYA function interchangeably, thus indicating that the Y chromosome does not harbor loci contributing to hybrid incompatibility. Therefore, Y chromosome gene flow between members of the gambiae complex is possible even at their current level of divergence. Importantly, this also suggests that malaria control interventions based on sex-distorting Y drive would be transferable, whether intentionally or contingent, between the major malaria vector species.